Methylene Blue
Methylene Blue is a phenothiazine dye that acts as a mitochondrial electron shuttle, with preliminary evidence suggesting potential benefits for cognitive enhancement, neuroprotection, & antimicrobial properties.
Evidence last reviewed: 05 Apr 2026
Not a routine supplement — not recommended for self-directed use.
Information here is educational only, not a recommendation to use. See our Safety page.
Evidence is from research or clinical settings — does not imply safety outside supervised contexts.
The evidence for Methylene Blue is primarily theoretical and low in strength, with some meta-analyses suggesting benefits in critically ill patients. However, the overall research is sparse, and more studies are needed to confirm its effectiveness and safety.
Hospital length of stayPatients with septic shock · Systematic reviewLow
Methylene Blue may reduce hospital length of stay in patients with septic shock.
Cognitive enhancementGeneral population · TheoreticalVery low
Methylene Blue is theorized to enhance cognitive function through its role as a mitochondrial electron shuttle.
NeuroprotectionGeneral population · TheoreticalVery low
Methylene Blue is theorized to offer neuroprotective benefits due to its antioxidant properties.
Mortality reductionCritically ill and perioperative patients · Meta-analysisLow
Methylene Blue may reduce mortality in critically ill & perioperative patients.
Vasopressor discontinuation timePatients with septic shock · Meta-analysisLow
Methylene Blue may reduce the time to vasopressor discontinuation & increase vasopressor-free days.
Cognitive & Energy Enhancement
Modafinil & methylene blue are combined to enhance cognitive function & energy levels. Modafinil is a wakefulness-promoting agent that improves focus, while methylene blue supports mitochondrial function & cognitive performance.
Cognitive & Neuroprotection Support
Pyrroloquinoline quinone (PQQ) & methylene blue are combined to support cognitive function & provide neuroprotection. PQQ supports mitochondrial function & cognitive performance, while methylene blue enhances mitochondrial function & provides neuroprotective benefits.